miscript hispec buffer (Qiagen)

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Qiagen miscript hispec buffer
Miscript Hispec Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/miscript+hispec+buffer/miscript+hispec+buffer/pmc12184905-94-14-12
Average 90 stars, based on 1 article reviews

miscript hispec buffer - by Bioz Stars, 2026-10

90/100 stars

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Article Title: Role of Protein Tyrosine Phosphatase Receptor Type E (PTPRE) in Chemoresistant Retinoblastoma.
Article Snippet: Real-time PCR reactions were conducted in triplicates in 20 μL of SYBRTM green PCR Mastermix (Applied Biosystem, Darmstadt, Germany) performing 40 cycles of the following program: 95 ◦C for 15 min, 94 ◦C for 15 s, 55 ◦C for 30 s and 70 ◦C for 34 s. For micro-RNA expression analyses, a miScript PCR Starter Kit (# 2181193; Qiagen, Hilden, Germany) was used, following the manufacturer ́s protocol. .. The designated miScript HiSpec Buffer (Qiagen, Hilden, Germany) for quantification of mature miRNA was used along with specific primers for miR631 5′-AGACCTGGCCCAGACCTCAGC-3′ and 5.8S RNA (5′-CTACGCCTGTCT GAGCGTCGCTT-3′) as an endogenous control. .. The reactions were performed in duplicates using a QuantStudioTM 3 real-time PCR system (Thermo Fisher, Darmstadt, Germany), with the following program: 95 ◦C for 15 min, 94 ◦C for 15 s, 55 ◦C for 30 s and 70 ◦C for 34 s and 40 cycles.

Article Title: Role of Protein Tyrosine Phosphatase Receptor Type E (PTPRE) in Chemoresistant Retinoblastoma
Article Snippet: Real-time PCR reactions were conducted in triplicates in 20 μL of SYBR TM green PCR Mastermix (Applied Biosystem, Darmstadt, Germany) performing 40 cycles of the following program: 95 °C for 15 min, 94 °C for 15 s, 55 °C for 30 s and 70 °C for 34 s. For micro-RNA expression analyses, a miScript PCR Starter Kit (# 2181193; Qiagen, Hilden, Germany) was used, following the manufacturer ́s protocol. .. The designated miScript HiSpec Buffer (Qiagen, Hilden, Germany) for quantification of mature miRNA was used along with specific primers for miR631 5′-AGACCTGGCCCAGACCTCAGC-3′ and 5.8S RNA (5′-CTACGCCTGTCT GAGCGTCGCTT-3′) as an endogenous control. .. The reactions were performed in duplicates using a QuantStudio TM 3 real-time PCR system (Thermo Fisher, Darmstadt, Germany), with the following program: 95 °C for 15 min, 94 °C for 15 s, 55 °C for 30 s and 70 °C for 34 s and 40 cycles.

Article Title: Gastric Inhibitory Polypeptide Receptor (GIPR) Overexpression Reduces the Tumorigenic Potential of Retinoblastoma Cells
Article Snippet: MiRNA expression analyses were conducted using a miScript PCR Starter Kit (cat. #2181193; Qiagen, Hilden, Germany) following the manufacturer’s instructions. .. For miRNA quantification, miScript HiSpec buffer (Qiagen, Hilden, Germany) was used with specific primers for miR-542-5p (5′-TCGGGGATCATCATGTCACGAGA-3′) and 5.8S RNA (5′-CTACGCCTGTCTGAGCGTCGCTT-3′) as an internal control. ..

Reverse Transcription:

Article Title: Expression Patterns of miRNAs in Egyptian Children with ADHD: Clinical Study with Correlation Analysis
Article Snippet: The RNA purity and integrity were screened by using a nanodrop spectrophotometer (2000 c, Thermoscientific, USA) and kept at -80 °C until further processing. .. Reverse-transcription was carried out for cDNA synthesis by using a miScript HiSpec buffer supplied in the miScript II RT Kit (Qiagen, Germany), following the supplier’s instructions. .. The expression profiles of the selected miRNAs were carried out using quantitative real-time polymerase chain reaction ( qRT-PCR ) (DNA Technology, Moscow, Russia) using miScript SYBR Green PCR master mix (Qiagen, Germany) with miScript primer assays (Qiagen, Germany) specific for the selected miR-138-1, miR-296-5p, miR-34c-3p, and miR-155.

Article Title: Circulating MicroRNAs Related to Arterial Stiffness in Adults with HIV Infection
Article Snippet: .. After elution, 1.5 μL of the RNA sample was used as a template for reverse transcription with the miScript II RT Kit (Qiagen) using the miScript HiSpec Buffer. ..

Article Title: repDilPCR: a tool for automated analysis of qPCR assays by the dilution-replicate method
Article Snippet: Total RNA was independently isolated from all replicates using the RNeasy Mini Kit (Qiagen, Hilden, Germany), including treatment with DNase. .. Reverse transcription to cDNA was carried out using the miScript II RT Kit with the miScript HiSpec buffer (Qiagen). ..

Article Title: Ameliorative effect of Astaxanthin on DMBA-induced breast cancer in female rats: Interplay between Notch-1 and related miRNAs.
Article Snippet: Instigating novel therapeutic strategies to combat breast cancer has become an urgent need.. Astaxanthin (ASX), a keto-carotenoid, has been confirmed to possess antitumor activity, yet studies on breast cancer are still limited.. The present study was deployed to unveil ASX’s antitumor effects, alone or combined with doxorubicin (DOX), on 7,12dimethylbenz(a)anthracene (DMBA)-induced mammary tumors in Sprague‒Dawley female rats.

cDNA Synthesis:

Transformation Assay:

Article Title: The bovine oviductal environment and composition are negatively affected by elevated body energy reserves
Article Snippet: .. Total RNA was transformed into cDNA using the miScript II RT Kit (QIAGEN) and miScript HiSpec Buffer to obtain mature miRNAs in OV-Cells and into miScript HiFlex Buffer to obtain mature and precursor miRNAs in OF-EVs, similar to previous methods [ ]. ..

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